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For a particularly interesting discussion have a look at this observation http://www.ispotnature.org/node/377693#comment-134339 which comes complete with excellent comments from Richard Comont (UKLadybird Survey) on distinguishing Harlequins. (Thanks Richard for that extremely useful input).
Remember to take a photo of the ventral side (underneath) of the ladybird as the ventral markings and leg colour can be critical in identification.
Also, scale (ie size) is critically important to aid identification.